When accessing a device via Splashtop, the user's audio will come through the link. While not really an issue - you may like thrash metal - it can be problematic if you are using VoIP to call them; the audio will cut off.
Here's what to do:
There is indeed an option you can enable in order to disable the sound from the Splashtop sessions.
You just need to open the Splashtop Streamer on the customer's computer->Settings->Sound->Tick 'Output sound on this computer only' - please see my below screenshot.


We could probably put that in a script and deploy to all clients.
"HKEY_LOCAL_MACHINE\SOFTWARE\WOW6432Node\Splashtop Inc.\Splashtop Remote Server\AutoMute" is changed from value 1 to 2.